Unformatted text preview: otide Pentanucleotide Hexanucleotide (CA)(CA)(CA)(CA) (GCC)(GCC)(GCC) (AATG)(AATG)(AATG) (AGAAA)(AGAAA) (AGTACA)(AGTACA) Require size based DNA separation to resolve different alleles; dinucleotide repeats require higher resolution Short Tandem Repeats (STRs)
AATG AATG AATG Summary of the Process of DNA Typing with STRs
%LRORJ\ 6DPSOH 2EWDLQHG IURP &ULPH 6FHQH RU 3DWHUQLW\ ,QYHVWLJDWLRQ '1$ '1$ ([WUDFWLRQ ([WUDFWLRQ '1$ '1$ 4XDQWLWDWLRQ 4XDQWLWDWLRQ 7HFKQRORJ\ 6HSDUDWLRQ DQG 'HWHFWLRQ RI 3&5 3URGXFWV 675 $OOHOHV 6DPSOH *HQRW\SH 'HWHUPLQDWLRQ 3&5 $PSOLILFDWLRQ 3&5 $PSOLILFDWLRQ RI 0XOWLSOH 675 PDUNHUV RI 0XOWLSOH 675 PDUNHUV 7 repeats 8 repeats the repeat region is variable between samples while the flanking regions where PCR primers bind are constant Homozygote = both alleles are the same length Heterozygote = alleles differ and can be resolved from one another Primer positions define PCR product size &RPSDULVRQ RI 6DPSOH *HQRW\SH WR 2WKHU 6DPSOH 5HVXOWV *HQHWLFV *HQHUDWLRQ RI &DVH 5HSRUW ZLWK 3UREDELOLW\ RI 5DQGRP 0DWFK Butler, Figure 1.2 ,I PDWFK RFFXUV FRPSDULVRQ RI '1$ SURILOH WR SRSXODWLRQ GDWDEDVHV Genotypes Genotype Designations The allelic combination in an individual that causes a particular trait AA, Aa, aa genotypes Allele = alternate form of a gene Genotypes can represent genes Genotypes can also represent alleles for non-protein coding segments for the DNA i.e. short tandem repeats )RU LQVWDQFH Individual 1 maternal allele AND 1 paternal allele 0RUH 7HUPLQRORJ\ Terminology Allele Locus Allele = alternate form of a gene Locus = the position or location of a gene on a chromosome
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This note was uploaded on 09/21/2009 for the course MCB 3416 taught by Professor Ladd during the Fall '08 term at UConn.
- Fall '08