MCB+102+F09+Lect+_6+POST[1]

MCB+102+F09+Lect+_6+POST[1] - Announcements Four Levels of...

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Announcements
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Four Levels of Structure in Proteins Primary Structure Secondary Structure Tertiary Structure Quaternary Structure
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AAAA Gene mRNA The purified bioactive molecule
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Primary Structure (Amino Acid Sequence) of Bovine Insulin A chain B chain N N C C
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Amino Acid Sequence Determination: disulfide
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O - H H + .. n Strong acid hydrolysis (6 N HCl, 110˚C, 24 hrs under vacuum) AA 1 2 3 6 AA n 4 AA 5 AA 8 AA 9 7 HOH HOH HOH HOH Hydrolyzate + + + +
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Stanford Moore ( left ) and William H. Stein ( right ) developed chromatographic the first automated amino acid analyzer in 1965 at Rockefeller University. They won the Nobel Prize in Chemistry in 1972. The First Automated AA Analyzer A Modern-Day AA Analyzer
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Dowex-50 (a sulfonated polystyrene resin)
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R— N H 2 Fluorescamine Fluorescent adduct N R— N H 2 Ruhemann's purple Ninhydrin - Detection of Free Amino Acids
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Amino Acid Sequence Determination: disulfide
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Determining the Amino-terminal Residue 1 2 3 4 5 6 7 8 9 10 11 12 13 15 16 17 18 19 14 20 Standards (20 DNP-AAs) 2D TLC
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This note was uploaded on 09/22/2009 for the course MCB 58168 taught by Professor Thorner during the Spring '09 term at Berkeley.

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MCB+102+F09+Lect+_6+POST[1] - Announcements Four Levels of...

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