notes - 4.21.09

notes - 4.21.09 - s of a transcription factor family MADS...

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s of a transcription factor family MADS (except leafy and apetala2 Biol 205 notes – 4.21.2009 Arabidopsis thaliana : model organism - Is a dichot - Small weed, can fit thousands of plants into a small growth room - Generation time = 6 weeks - Intensely studied for past 15 years, excellent genetic and experimental tools developed - Genome = 125 Mb (SMALL) o First plant genome sequenced - Advantage: can self-fertilize (for genetics) - Finding mutants: 1. Mutagenize seeds (M1) 2. Grow M1 plants and allow to self-fertilize and collect M2 seed 3. Screen m2 plants for mutants Mutant       x Mutant + + ¼ mutant/mutant, ½ mutant/+, ¼ +/+ Mutant       x + + + Mutant/+ x Mutant/+    results above    Agrobacteria infect plants - Cause huge tumors - Function by transfer a linear piece of DNA from their plasmid (T-DNA) - Inserts randomly somewhere on plant chromosome Agrobacterium-mediated plant transformation - Use function of agrbacteria to put target gene into plant - Gene providing drug resistance (marker) + whatever target gene -  Transgenic plant Differences in development with plants: - Plants do NOT undergo gastrulation. 
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- Have rigid cell walls, so cells don’t move around. Must generate tissue types solely by cell 
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notes - 4.21.09 - s of a transcription factor family MADS...

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