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Lab Manual - BIOLOGY 1 AL Laboratory Manual UC Berkeley...

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BIOLOGY 1 AL Laboratory Manual UC Berkeley 2009/2010 Lab Lectures are mandatory! Come to the first lab lecture having prepared. You can download the syllabus from our website. The url is mcb.berkeley.edu/courses/bio1a . Complete the pre-lab prior to lab. The pre-labs and lab worksheets are available at our website. This will be a very useful website. At the start of each lab there will be a quiz worth five points. It will cover the assigned reading and material from the lab lecture. The quiz will start at 10 or 40 minutes after the hour, depending on your lab time. If you are late, then you will miss the quiz. There are no make-up quizzes nor are there make-up labs. Please write your name and contact information below as we have a surprisingly large number of lab manuals left in lab. Name: . Section #: Contact Information: Ruler Calibration:Total mag. 100X = , 400X = . (from p. 26)
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Table of Contents – listed by topic. TABLE OF CONTENTS LISTED BY TOPICS (refer to your syllabus) INTRODUCTION 1-4 Course Overview, Animal use, grade and point breakdown 1-4 SAFETY 5-10 Lab Safety 5-8 Lab Supplies/Design 9-10 EQUIPMENT USE 11-28 Spectrophotometer 11-14 Micropipetter 15-18 Compound Microscope 19-28 CELLS 29-42 Cell Theory 29-30 Prokaryotes 30-33 Moneran overview 30-32 Cyanobacteria 33 Eukaryotes Eukaryote overview 34-35 Plantae 36-37 Protists 38-42 Glossary 43-44 CHORDATE DIVERSITY 45-63 Chordata/Vertebrata Evolution 45-52 Fishes 53-55 Amphibians 56-58 Amniota 58 Reptiles/Birds 59-61 Mammals 62-63 CONSTRUCTING RECOMBINANT DNA 64-90 Summary diagram of constructing recombinant DNA 64 Overview 64-67 Ligation reactions 66-68 Transformation 69-76 Isolation and culturing of appropriate transformed E. coli 77-78 Plasmid DNA isolation and DNA digestion (done in GMB I lab) 79-84 DNA electrophoresis (done in GMB II lab) 85-88 Summary diagram of constructing recombinant DNA 64 Glossary of genetic terms 89-90 ENZYMES 91-97 Enyzme lab 91-98 Tutorial: Working with enzymes 99-110 COMPLEMENTATION 111-118 Complementation – part 1. Streaking onto YPD plates. 111-113 Complementation – part 2. Replica plating onto SD plates. 114-116 Complementation – part 3. Analyzing growth. 116-118
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Table of Contents – listed by topic. PHOTOSYNTHESIS 119-133 Photosynthesis lab 119-132 Tutorial: Photosynthesis 133 GENETICS AND MOLECULAR BIOLOGY I 135-144 Mitosis 135-136 Meiosis 137-140 DNA Sequencing 141-144 GENETICS AND MOLECULAR BIOLOGY II 145-158 Mendelian Genetics in Drosophila 145-156 Dissecting Microscope Use 157-158 BIOINFORMATICS On-line only LAB EXAM I: See syllabus. INTRODUCTION 159-164 Use of Animals, Introduction to animal diversity, Cladistics 159-164 MORPHOLOGY AND DESCRIPTIVE TERMS 165-166 VERTEBRATE ANATOMY 167-176 Rat Dissection 167-176 INVERTEBRATE I 177-192 Porifera 177 Cnidarians 178-179 Platyhelminthes 180-181 Annelids 182-186 Molluscs 187-191 Glossary 191-192 INVERTEBRATE II 193-205 Echinodermata 193-195 Arthropods 196-204 Glossary 205 REPRODUCTION & DEVELOPMENT 207-215 Introduction: Protostomes vs. Deuterostomes 207-208 Arthropods 208 Gametogenesis 209-210 Fertilization and Embryonic Development 211-216 Larval Development 216 Glossary 218 RESEARCH OPPORTUNITIES 219-220 LAB EXAM II: See syllabus. Held during your 3 hour lab.
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