Histology%20Slides - Use this file as a primer(of sorts on...

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Unformatted text preview: Use this file as a primer (of sorts) on histological examples. taken from http://pharm.shams.edu.eg/Histology%20slides.pdf Histology slides Epithelial tissue 1) Simple squamous epithelium Section of Bowman’s capsule in the kidney (H&E) Flat epithelial cells with flat nuclei Each little purple circle is a deeply stained cell nucleus. Being that we're looking at epithelial tissue, we can assume each purple cot (i.e. nucleus) represents a single individual cell. Note: These structures are NOT single cells... they are a special type of vessel. 2) Simple cubical epithelium Section of thyroid gland (H&E) Cubical cells with central nuclei Glandular tissue provides an example of epithelial tissue... how would we structurally classify this tissue? 3) Simple columnar epithelium Section of gall bladder (H&E) Columnar tall cells with oval basal nuclei Note the single layer. Can you tell which cells are goblet cells? 3) Pseudostratified columnar ciliated epithelium Section of the trachea (H&E) Cells appear to form several layers. All cells reach the basement membrane, but not all of them reach the surface. Motile cilia cover cell apices except those of the light staining oval goblet cells. The deep[r nuclei belong to the short basal cells, and the more superficial oval nuclei belong to the columnar ciliated cells. Each luminal epithelial cell is attached to the associated basement membrane. You should be able to identify and label the various tissue types represented in this slide. Pseudostratified columnar ciliated epithelium 4) Stratified squamous non-keratinized epithelium Section of a thin skin (H&E) The epithelium is formed of several layers; the basal is columnar cells, the middle layers are polygonal cells with central round nuclei, and the superficial ones are flat squamous cells with flattened nuclei 5) Stratified squamous keratinized epithelium Section of a thick skin (H&E) The epithelium is formed of several layers; the basal is columnar cells, the middle layers are polygonal cells with central round nuclei, and the superficial ones are flat squamous cells with flattened nuclei. There is a surface horny layer formed of keratin cells 6) Transtional epithelium Section of the urinary bladder (H&E) The epithelium is formed of several layers; the basal is columnar cells, the middle layers are polygonal cells with central round nuclei, and the superficial ones are cubical cells with rounded nuclei. Some cells are binucleate Where in the body can one be sure to find transitional epithelium? 7) Glandular cells Section of the pancreas (H&E) The pancreas contains an exocrine portion formed of acini and ducts, and an endocrine one represented by the islets of Langerhans. Pancreatic acini are lined by pyramidal cells with spherical nuclei, and basophilic cytoplasm. STOP! Do some researching, and find out what the different functions of alpha, beta, and delta cells are within the pancreas. Connective tissue 8) Mucous connective tissue Section of an umbilical cord with two arteries and a vein Look how far apart these purple dots are... What do purple dots mean, again? How is this different from the slides we saw of epithelial cells? Mucoid connective tissue. Arrow points to fibroblasts immersed in an extraxellular matrix (M) 9) Fibrous connective tissue Note how much matrix material there is surrounding the cellular mass, even in this regular dense connective tissue. 10) Adipose connective tissue STOP! Remember from Bio 1 !!! Why is it better to store energy as lipids instead of carbohydrates... other than the fact that lipids store MORE energy than car 10) Elastic connective tissue Ligamentum nuchea STOP! Why is it so difficult for a ligament to heal after it's been torn? Aorta (orcein) These are cellular layers associated with the major arteries... (aka "tunicae")... don't worry about remembering these. Aorta (H&E) 11) Reticular connective tissue Lymph node silver Reticular fibers (arrows) in lymph node Reticular fibers in a lymph node (Silver) (silver preparation). A silver stain is another staining method... no, you can't melt down your lymph nodes and pawn them off. Cartilage &bone 12) Hyaline cartilage (trachea, costal caertilage) Costal cartilage Trachea 13) Elastic cartilage (Ear pinna) STOP! Label the lumen of the trachea. In these slides, note that bone cells are built along very particular stress lines. When bone breaks, it is more likely to fracture ALONG these stress lines unless the injury resulted from a crushing blow. STOP! Draw in the stress lines in the longitudinal section slide. 14) Ground dense bone (L.S &T.S) Transverse section Compact bone Longitudinal section 15) Cancellous bone STOP! Can you draw the stress lines in the above slides as well? If not, why? STOP! Come up with at least five differences between cancellous and compact bone. Now, come up with five similarities! Cancellous bone 16) Developimg bone STOP! Describe the characteristics of 1) Liquid 2) Gelatinous 3) Solid connective tissue matrices and list the areas of the body you're likely to find them. Blood 18) Blood film of man - Note: Don't worry about committing to memory the various polymorphonuclear leukocytes. (and yes... that IS a word!) Muscle 19) Skeletal muscle fibres (L.S &T.S) STOP! How many nuclei does each skeletal muscle fiber have? 20) Smooth muscle fibres STOP! You're a lab technician wanting to selectively label smooth muscle cells amidst a bunch of skeletal muscle fibers with a fluorescing antibody. What special protein might you target? (Hint... think ion channels) 21) Cardiac muscle fibers Nervous tissue 22) Nerve trunk (H& E) 23) Nerve trunk (osmic acid) 24) Spinal ganglion (H& E) Note all the different tissue types represented in this slide. Spinal ganglion (silver) 25) Dorsal root ganglion (left) and sympathetic ganglion (right) (H& E) Sympathetic ganglion Histologically speaking, this slide contains many terms beyond the scope of this course. Cardiovascular system 27) Aorta Again with the tunicae! 28) Large vein 26) Medium sized artery 28) Medium sized vein STOP! What are some characteristics that would allow you to differentiate between a vein and an artery? 29) Artery and vein Lymphatic system 32) Lymph node 33) Spleen 34) Thymus 35) Tonsils Skin 36) Thin skin 37) Thick skin Digestive system 38) Liver pig (H& E) 39) Liver human (H& E) Urinary system 40) Mammalian kidney 41) Human kidney 1) Kidney Thyroid gland Tall columnar ciliated epithelium (trachea) 4) Thick Skin Epidermis of skin Urinary bladder Umbilical cord 7) Pancreas Mucoid C.T. 10) Tendon (longitudinal section) Tendon (transverse section) Adipose C.T. 13) Hyaline cartilage (trachea) Cells and matrix of hyaline cartilage Elastic cartilage 16) Developing bone (intracartilaginous ossification) Cancellous bone Compact bone (transverse section) 19) Compact bone (longitudinal section) Compact bone (osteon) Blood film 22) Smooth muscle Skeletal muscle Cardiac muscle 25) Skeletal muscle Cardiac muscle Peripheral nerve & blood vessels (transverse section) 28)Nerve (longitudinal section) Nerve (osmic acid) Spinal ganglion 31) Spinal ganglion Spinal ganglion Sympathetic ganglion 34) Spinal ganglion (silver) Spinal ganglion (silver) Aorta (H&E) 37) Arota (orecin) Artery and vein Large vein 40) Lymph node Thymus Spleen 43) Tonsil Thin skin Thick skin 46) Liver (pig) Liver (human) Liver human 49) Kidney Kidney (Cortex) Kidney (cortex &medulla) ...
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This note was uploaded on 09/25/2009 for the course BIOSC 1080 taught by Professor Roberets during the Summer '07 term at Pittsburgh.

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