Midterm%20exam%203%20answers

Midterm%20exam%203%20answers - BIOSC 0350 Spring Term 2084...

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BIOSC 0350 Name: ANSWER KEY Spring Term 2084 Third Mid-Term Exam Signature: April 10, 2008 Dr. Valerie Oke INSTRUCTIONS: 1. Print your name at the top of each page and make sure you have 12 exam pages and one page of scrap paper. 2. Sign the first page of this examination in the space provided. 3. Take a few moments to look over the entire exam before answering any questions. Then, begin with the questions you are most comfortable answering. 4. Show your work. In some cases partial credit will be given. Write your final answers and work, including calculations if applicable, in the boxes or on the lines provided. 5. Please write legibly. Make your drawings clear. Be concise; you will not be given credit for extraneous information. 6. Some of the model systems described in this exam parallel actual genetic systems but are solely derived from the imagination of the instructors. 7. Remember to relax and do your best. Good Luck!! Print
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Name Page 2 of 12 BIOSC 0350 Mid-Term Exam 3 April 10, 2008 Question Possible Points Score 1 17 2 18 3 22 4 14 5 15 6 14 Total 100
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Name Page 3 of 12 BIOSC 0350 Mid-Term Exam 3 April 10, 2008 1) 17 points total. You are studying a disease in mice called MOD. Affected mice are homozygous for a recessive allele ( mod - ) of the mod gene. In order to clone and sequence the mod gene, you must first map the gene. You identify a restriction fragment length polymorphism closely linked to mod in which the DNA probe hybridizes to a 10-kb fragment in the inbred strain C3H, and hybridizes to two fragments, 7 kb and 3 kb, in the inbred strain DBA. A cross is carried out between a pure-breeding female from the C3H strain affected by the MOD disease and a pure-breeding male from the DBA strain unaffected by disease. The F 1 males and females (brother x sister) are crossed to produce an F 2 that includes 16 individuals in their first litter. The results of this cross are shown in the following pedigree: a) 10 points. What are the genotypes of the following numbered mice? If more than one genotype is possible (excluding new mutations), indicate the alternatives. Include both the mod and RFLP genotypes ( mod + vs. mod - and 10 vs. 7+3) in your answer and show correct linkage on the chromosomes. Note that the Southern blot lanes do not line up with the pedigree but are labeled by individual at the bottom. I #1: III #9: I #2: III #11: II #3: III #16: III #18: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 3+7 mod+ / 3+7 mod+ or 3+7 mod- / 3+7 mod+ 10 mod- / 10 mod- 3+7 mod+ / 10 mod- or 3+7 mod+ / 10 mod+ or 3+7 mod- / 10 mod+ 3+7 mod- / 10 mod- 10 mod- / 10 mod- 3+7 mod+ / 3+7 mod+ 10 mod- / 3+7 mod+ 10 mod- / 3+7 mod+
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Name Page 4 of 12 BIOSC 0350 Mid-Term Exam 3 April 10, 2008 1) Continued. b) 5 points. Additional F 2 mice were obtained from matings of the F 1 mice. The table below shows the RFLP phenotypes observed for 60 diseased mice: RFLP fragments No. of mod diseased mice 10 kb 55 parental 10, 7, and 3 kb 4 recombinant, one crossover between one pair of homologous chromosomes 7 and 3 kb 1 recombinant, two crossovers total, one between each pair of homologous chromosome What is the distance in map units from the RFLP to the mod
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This note was uploaded on 09/25/2009 for the course BIOSC 350 taught by Professor Martens during the Spring '07 term at Pittsburgh.

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Midterm%20exam%203%20answers - BIOSC 0350 Spring Term 2084...

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