Lecture 10 final

Lecture 10 final - Prokaryotic
Gene
Regulation
...

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Unformatted text preview: Prokaryotic
Gene
Regulation
 I.
Introduction
 
 
A.
Evidence
of
requirement
for
regulation
 II.
Protein/DNA
interactions
 
 A.
Positive
regulation
 
 B.
Negative
regulation

 III.
Operon
 
 A.
Lactose
–
inducible
system

 
 
1.
Regulation
in
wild
type
 
 
2.
Mutational
analysis
 
 B.
Tryptophan
–
repressible
system
 IV.
Recombinant
DNA
cloning
based
on
lac
operon
 

































Prokaryotic
Gene
Regulation
 Objectives:
 Understand
positive
vs
negative
regulation,
and
the
concept
of
the
operon
as
a
regulatory
unit
 Understand
the
components
of
the
lac
operon

 Know
the
difference
between
cis
and
trans
acting
factors
 Be
able
to
determine
genotypes
and
phenotypes
of
different
combinations
of
lac
operon
mutations
 Be
able
to
construct
and
interpret
recombinant
DNA
plasmids
that
utilize
the
lac
system

 Regulatory proteins control transcription The lac operon is transcribed only in the presence of lactose Figure
10‐6
step
4
 Repressor
structure
 Glucose levels control the lac operon Figure
10‐13
step
3
 Negative and positive control of the lac operon Figure
10‐17
step
3
 Assignment

 Achieve
Objectives
List
(slide
2)
through
study
 and
practice.
 Solve
problems
from
textbook.

 Chapter
10:
 1,
2,
4,
12
 ...
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