Lecture 11 Guide

Lecture 11 Guide - Eukaryotic
Gene
Regulation


Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Eukaryotic
Gene
Regulation
 I.
Complex
regulation:
Multiple
levels
and
types
 II.
Levels
and
types,
with
some
examples
 
 A.
Chromatin
and
DNA
 
 
1.
Heterochromatin,
euchromatin
 
 
 
a.
X
inactivation
 
 
 
b.
Position
effect
variegation

 
 
2.
DNA
methylation
 
 
 
a.
Imprinting
 
 
2.
DNA
rearrangement
 
 
 
a.
Antibody
genes
 
 
3.
Multiple
genes
 
 
 
a.
Hemoglobin
 
 B.
Transcription

 
 
1.
Promoter
 
 
2.
Enhancers,
enhanceosome
 
 
3.
Transcription
factors
 
 
 
a.
Binding
motifs
 
 
4.
Yeast
Gal4
system
 
 C.
Post‐transcription
 
 
1.
Alternative
splicing
 
 
2.
Regulation
by
micro
RNA
 
 D.
Translation
and
post‐translation
 

































Eukaryotic
Gene
Regulation
 Objectives:
 Be
able
to
answer
the
following
study
questions:
 
 
 
 Why
are
there
so
many
ways
to
regulate
eukaryotic
gene
expression,
and
how
do
these
work?
 What
is
the
significance
of
structural
motifs
found
for
transcription
factors?
 What
is
the
difference
between
a
promoter
and
an
enhancer?
 
 Define
and
describe
how
each
term/system
in
the
vocabulary
list
works,
and
give
an
example
 of
each.
 
 
 What
does
it
mean
to
regulate
in
cis
versus
in
trans?
 
What
is
similar
and
what
is
different
compared
to
gene
regulation
in
prokaryotes? Vocabulary genomic imprinting DNA methylation yeast Gal4 system upstream activation sequence chromatin remodeling histone acetylation euchromatin heterochromatin position effect variegation enhancer insulator DNAse hypersensitive transcription factor motifs epigenetic inheritance tissue and developmental specificity microRNA Modified histone tails protrude from the nucleosome Figure 11-12 Gene silencing is caused by the spread of heterochromatin Figure 11-23 Steps required for imprinting Figure 11-21 Overview of transcriptional regulation Figure 11-2 Promoter-proximal elements precede the promoter of a eukaryotic gene Figure 11-3 Variations on the theme of hemoglobin Figure 19-17 Hemoglobin genes duplicated; some evolved into nonfunctional pseudogenes (ψα and ψβ ) Figure 19-18 Table 19-4 Enhanceosomes help recruit the transcriptional machinery Figure 11-14 Enhanceosomes recruit chromatin remodelers Figure 11-15 Transcriptional activator proteins bind to UAS elements in yeast Figure 11-6 Transcriptional activator proteins are modular Figure 11-7 Transcriptional activator proteins may be activated by an inducer Figure 11-8 A microRNA controls developmental timing Figure 12-25 Assignment

 Achieve
Objectives
List
(slide
2)
through
study
 and
practice.
 Solve
problems
from
textbook.

 Chapter
11
 3,
5,
10,
11,
12,
14,
16,
17,
18
 ...
View Full Document

Ask a homework question - tutors are online