Lecture 12 Guide

Lecture 12 guide

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Unformatted text preview: Recombinant
DNA
Applications
 I.
Construction
of
recombinant
DNA
 
 A.
Libraries
 
 
 
1.
Genomic
 
 
 
2.
cDNA
 
 B.
Screening
 II.
Molecular
genetic
diagnostics
 
 A.
RFLP
 
 B.
DNA
fingerprinting
 III.
Genetic
engineering
 
 A.
Production
of
transgenic
organisms
 
 
1.
Various
organisms
and
approaches
 
 
2.
Focus
on
mouse
transgenics
 IV.
Comprehensive
example:
globin
genes
 

































Eukaryotic
Gene
Regulation
 Objectives:
 Know
how
to
construct
and
screen
plasmid
and
phage
libraries
 Be
able
to
interpret
RFLP
and
DNA
fingerprinting
data
 Understand
the
uses
of
recombinant
DNA
libraries
and
transgenic
organisms
 Starting
with
a
cloned
gene,
be
able
to
delineate
the
production
of
transgenic
mice
 Vocabulary ( mainly review) forward genetics cDNA library autoradiogram dideoxy sequencing PCR donor and vector DNA gene knockout gene replacement functional complementation Southern and northern blotting somatic gene therapy germ line gene therapy hybridization western A plasmid vector, pUC18 Construction
of
cDNA
 Finding the clone of interest by using antibody Figure
20‐10
 Cloning in phage λ Figure
20‐6
 Finding the clone of interest by using DNA...
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This note was uploaded on 09/26/2009 for the course BIS 98659 taught by Professor Kimbrell during the Summer '09 term at UC Davis.

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