Lectures 8 and 9

Lectures 8 and 9 - I.Centraldogma II.Transcription...

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Transcription and Translation I. Central dogma II. Transcription A. Initiation, Elongation and Termination B. Prokaryotes vs. Eukaryotes III. Translation A. Initiation, Elongation and Termination B. Prokaryotes vs. Eukaryotes IV. Mutations A. DNA ‐> RNA ‐> protein V. Methods A. Northern B. Western
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Genetics of Bacteria and their Viruses Objectives: Know key features of transcription and translation in prokaryotes and eukaryotes Know the events involved in processing eukaryotic mRNA and the role of processing Be able to track the effects of mutations through to effect on protein sequence and phenotypes
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Vocabulary: transcription promoter consensus sequence monocistronic/polycistronic RNA polymerases I, II, III RNA processing G cap poly A tail colinear intron/exon sigma factor holoenzyme hairpin loop catalytic RNA tRNA rRNA ribosome amino‐acyl tRNA synthetase A and P sites codon anticodon wobble degenerate code start codon, stop codon reading frame non‐overlapping colinearity of gene and protein
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Lectures 8 and 9 - I.Centraldogma II.Transcription...

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