midterm 2 - 1. Prokaryotes: class for bacteria. 2. Viruses:...

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1. Prokaryotes: class for bacteria. 2. Viruses: parasitizes bacteria. 3. Phage recombination: when two or more phages of different genotypes both infect the same bacterial cell. 4. Plating: a small amount of liquid culture is spread out into solid agar medium. 5. Colony: mass cells seen with a naked eye on an agar plate 6. Cell clone: members of a colony that have a single genetic ancestor. 7. Prototrophic: grow and divide on minimum medium. 8. Auxotrophic: mutants that can’t grow on minimal medium. 9. Resistant mutants: can divide and form colonies in the presence of the inhibitor. 10. Genetic marker: (marker alleles) to keep track of genomes and cells in experiments. 11. Conjugation: the physical union of bacterial cells can be confirmed under an electron microscope. 12. Donor: one parent and the only parent seemed to transfer some or all of its genome into another cell. 13. Recipient: receives the DNA. 14. Fertilization factor (F): strains that carry F can donate and are designated F+, and F- cannot donate. 15. Plasmid: a small, nonessential circular DNA molecule. 16. Rolling circle replication: the circular plasmid rolls and as it turns; it reels out the single stranded copy like a fishing line. 17. Hfr strain: the ability for F- strain to promote high frequency of recombination.
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18. Interrupted mating: a technique used to map bacterial genes by determining the sequence in which donor genes enter recipient cells. 19. Exconjugant: a female bacterial cell that has just been in conjugation with a male
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This note was uploaded on 09/26/2009 for the course BIS 98659 taught by Professor Kimbrell during the Summer '09 term at UC Davis.

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midterm 2 - 1. Prokaryotes: class for bacteria. 2. Viruses:...

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