Questions Part 3 b

Questions Part 3 b - Question 6. 


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Unformatted text preview: Question 6. 
 In
a
generalized
transduction
experiment,
the
T1
phage
particles
 growing
on
E.
coli
with
the
genotype
val+
ala+
trp+
are
collected
and
 allowed
to
infect
E.
coli
cells
with
the
genotype
val‐
ala‐
trp‐.


 
 The
cotransduction
frequencies
calculated
for
the
different
gene
 combinations
are: 

 
 
ala‐trp
=
0.2
 
 
ala‐val
=
0.8
 
 
val‐trp
=
0.6
 
 
 
 
 What
is
the
gene
order?
 
A.
ala
‐
trp
‐
val
 
B.
ala
‐
val
‐
trp
 
C.
val
‐
ala
‐
trp
 1
 Question 7. 
 You
have
obtained
a
plasmid
DNA
sample
from
a
friend,
but
he
is
not
sure
 whether
the
DNA
is
circular
(uncut)
or
has
been
linearized.

The
plasmid
should
 be
10kb
in
length
and
your
friend
tells
you
that
the
restrictions
enzymes
 BamHI,
EcoRI,
and
HindIII
each
cut
this
DNA
once.


 
 You
perform
restriction
enzyme
digests
of
the
DNA
and
obtain
the
 following
results
(length
of
resulting
DNA
fragments):
 HindIII:

7kb
+
3kb
 EcoRI:


8kb
+
2kb
 Is
the
plasmid
DNA:


A.

Circular
 
 
 
 

 
B.

Linear 

 2
 Question 8. 
 When
you
use
BamHI,
the
same
10kb
fragment
appears
that
you
 originally
had.

You
perform
control
experiments
with
other
DNA
 samples
with
known
BamHI
sites
and
you
generate
the
expected
 fragments.

You
conclude:
 
 
 A.

The
BamHI
is
not
active
 B.

The
DNA
has
already
been
linearized
with
BamHI
 
 C.

Your
friend
was
wrong,
there
is
no
BamHI
site
in
the

 






 plasmid.
 3
 Question 9. 
 You
perform
a
HindIII‐EcoRI
double
digest
with
your
friend’s
DNA
 and
obtain
three
fragments:

2kb
+
3kb
+
5kb.


 
 A.
 Which
below
is
the
correct
circular
map?

 HindIII 3kb BamHI 5kb EcoRI C.
 5kb HindIII 2kb BamHI 3kb B.
 BamHI 3kb EcoRI 5kb Remember
 2kb EcoRI 2kb HindIII HindIII:

7kb
+
3kb
 EcoRI:


8kb
+
2kb
 4
 ...
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