Questions Part 4 8 31

Questions Part 4 8 31 - Question 1...

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Unformatted text preview: Question 1. What
is
the
goal
of
sequencing
a
genome?
 Question 2. 
 Now
that
the
sequence
of
the
entire
human
 genome
has
been
obtained,
how
does
it
relate
to
 your
genomic
sequence?

 Question 3. 
 What
can
the
sequence
of
the
human
genome
 tell
us
about
the
basic
biology
of
humans?

 Question 4. How
many
chromosomes
are
involved
in
the
 human
genome
when
it
is
expressed
as
 “approximately
3
billion
base
pairs”?


 
 What
is
the
proper
name
for
this
type
of
genetic
 content?
 
 Question 5. 
 A
300
bp
open
reading
frame
(ORF)
has
the
 potential
to
encode
______
amino
acids
in
a
row.
 a.



10
 b.



30
 c.


100
 d.

300
 e.

900
 Question 6. Rank
from
“roughest ”
→
“fine
detail”
the
amount
 of
resolution
allowed
by
the
following
methods
of
 mapping:
 
 A.
Restriction
map




 B.
Cytogenetic
map





 C.
Linkage
map





 D.
Sequence
map
 Use
only
the
letter:
 Question 7. 
 Microarray
technology
directly
involves:
 a.
PCR
 b.
DNA
sequencing
 c.
Hybridization
 d.
RFLP
detection
 e.
None
of
the
above
 Question 8. 
You
wish
to
probe
a
human
cDNA
library
to
find
out
 which
insert
has
the
β‐globin
gene.


 
 
Which
of
the
following
would
be
a
good
choice
as
 your
probe
(assume
in
all
cases
your
probe
is
labeled)?
 
 a.
A
30b
oligonucleotide

corresponding
to
intron
1
 b.
A
30b

oligonucleotide

corresponding
to
exon
1
 c.
A
30
nucleotide
oligo
corresponding
to
a
β‐globin
gene
 enhancer
 d.
All
of
the
above
 e.
None
of
the
above
 Question 9. 
 In
using
bioinformatics
tools
to
search
for
possible
genes
in
the
DNA
 sequence
of
the
human
genome,
which
of
the
following
would
be
the
 stongest
evidence
for
a
region
being
genic,
i.e.
protein
encoding?
 a.
Presence
of
short
(3‐30
bp)
ORF
flanked
by
a
“start ”
and
“stop”
codon
 b.
Presence
of
a
long
(100
bp
or
more)
ORF
flanked
by
a
“start ”
and
 “stop”
codon
 c.
Presence
of
an
ATG
codon
(since
the
“start ”
codon
is
invariably
ATG).
 d.
All
of
the
above
 e.
None
of
the
above
 Question 10. 
 
 
Which
of
the
following
is
strong
evidence
that
a
putative
gene
 identified
by
sequence
analysis
of
the
genome
functions
as
a
bona
fide
 protein
encoding
gene
in
vivo?
 a.  Presence
of
introns
and
exons
in
the
genomic
sequence
 b.

Presence
of
a
“start ”
and
“stop”
codon
in
the
genomic
sequence
 flanking
relatively
long
ORFs
interrupted
by
intron
sequences
 c.

Presence
of
mRNA
in
the
cell
with
an
ORF
that
corresponds
precisely
 with
the
predicted
exons
in
the
putative
gene
 d.  All
of
the
above
 e.

None
of
the
above

 Question 11. 
 human
homolog
of
a
yeast
gene.
You
determine
the
DNA
sequence
 of
cDNAs
of
both
your
yeast
gene
and
the
human
gene
and
decide
to
 compare
the
gene
sequences,
as
well
as
the
predicted
protein
 sequence
of
each,
using
alignment
software.


 
 You
would
expect
the
greatest
sequence
identity
from
 comparisons
of
the:
 a.
cDNA
sequences
 b.
Protein
sequences
 c.
Genomic
DNA
sequences
 d.
Both
(a)
and
(b)
will
give
you
equivalent
sequence
similarity
 e.All
will
give
equivalent
sequence
similarity
 
 
You
are
a
researcher
who
has
tentatively
identified
a
 ...
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