Sample Final Exam

Sample Final Exam - Name: _ ID: _ 1. You are working on a...

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Name: ______________________________ ID: _______________________________ 1 1. You are working on a wolf genomics project, and are going to make a genomic DNA library of wolf DNA using the vector on the right. a. (10 pts) List the steps you would take to construct this library. What drug(s) will the plasmids without inserts grow on? What drug(s) will the plasmids with inserts grow on? Hind III Hind II Eco R I tet r kan r Hind III b. (4 pts) The gene for a particular peptide hormone has been cloned from dogs. Assuming that wolves have a very similar gene, how would you use this dog gene clone and your wolf DNA library to identify the wolf gene?
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Name: ______________________________ ID: _______________________________ 2 2. You are studying nutrition, and your project is to find out the effects of knocking out the function of a particular digestive enzyme gene in mice. You have already cloned the wild type mouse gene for this enzyme, designated gene D. a. (10 pts) List the steps you would perform to produce embryonic stem cells carrying a targeted mutation in gene D. What is the purpose of neomycin? What is the purpose of ganciclovir? b. (4 pts) Briefly describe how you would use the embryonic stem cells derived in part a to produce a transgenic mouse carrying the gene D mutation.
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Name: ______________________________ ID: _______________________________ 3 3. (8 pts) You are restriction mapping a fragment of DNA from a newly identified fungus. You have cleaved this linear piece of DNA with various restriction enzymes and obtained the following sizes of DNA: Enzyme(s) Fragment sizes, in kilobases BamHI 2, 9 SmaI 4, 7 HaeII 1, 10 BamHI and SmaI 2, 4, 5 BamHI and HaeII 1, 2, 8 SmaI and HaeII 1, 3, 7 Draw the restriction map of this DNA. 4. (6 pts) The following DNA fingerprinting result was obtained in the case of a paternity dispute. mother child male-1 male-2 a. Which person appears to be the father? _____ _____ _____ _____ b. Assuming half of the bands were _____ _____ A _____ _____ inherited from each parent, which _____ person did the child inherit band A _____ from? _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____ _____
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Name: ______________________________ ID: _______________________________ 4 5. (4 pts) Cloned genomic DNA from an organism has been mapped to a region of its X-chromosome, designated as 1-10 below. A-H represent the cloned DNA fragments and their relative locations. For example, A is a clone of the DNA in X-chromosome regions 1 and 2. 1
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Sample Final Exam - Name: _ ID: _ 1. You are working on a...

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