Sample Midterm 2 _some 1_

Sample Midterm 2 _some 1_ - Name: _ ID: _ 1 . You are...

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Name: ______________________________ ID: _______________________________ 2 1 . You are working on a wolf genomics project, and are going to make a genomic DNA library of wolf DNA using the vector on the right. a. (10 pts) List the steps you would take to construct this library. What drug(s) will the plasmids without inserts grow on? HindIII HindIII What drug(s) will the plasmids with inserts grow on? kan r HindIII EcoRI tet r b. (4 pts) The gene for a particular peptide hormone has been cloned from dogs. Assuming that wolves have a very similar gene, how would you use this dog gene clone and your wolf DNA library to identify the wolf gene?
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Name: ______________________________ ID: _______________________________ 3 2 . For each E. coli strain containing the lac operon alleles listed, indicate whether the strain is inducible, constitutive or unable to express B- galactosidase and permease. (10) B- galactosidase / permease a. i + p + o + z - y + /i + p + o c z + y + / b. i + p + o + z + y + /i - p + o c z + y - / c. i + p + o + z - y + /i - p + o + z + y - / d. i - p - o + z + y - /i + p + o c z - y + / e. i s p + o + z + y + /i - p + o + z + y - / 3. You are interested in how the nervous system functions, and have taken a position as a research intern in a lab that studies Drosophila. Other students before you have made a strain of flies that has an enhancer trap inserted 10 bases 5’ of the start codon of a gene that is expressed only in the brain. Your project is to clone the 5’ region, so that this DNA containing brain-specific enhancers can be further analyzed. How do you do this? Start by drawing the enhancer trap and surrounding DNA, indicating the
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Sample Midterm 2 _some 1_ - Name: _ ID: _ 1 . You are...

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