Sample Midterm 2 _some 1_ Answers

Sample Midterm 2 _some 1_ Answers - Name: _ ID: _ 1. a. -...

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Name: ______________________________ ID: _______________________________ 1 1 . a. - cut wolf DNA and plasmid DNA with EcoRI, - mix together and ligate the wolf DNA and plasmid DNA fragments, - transform the ligated DNA into bacteria (that are kan s and tet s ) - grow on kan to select for bacteria that have a plasmid - of these bacteria, those that cannot grow on tet are the bacteria containing plasmids with an insert of wolf DNA b. Identify the wolf DNA clone(s) that are homologous to the dog gene by colony hybridization using the dog gene as a probe. 2 .a, constitutive / constitutive b. constitutive / inducible c. inducible / inducible d. no expression / constitutive e . no expression / no expression 3. BglII P P/BglII / / weak promoter-lacZ w+ ampR / . en en (CAAT) TATA ------------ ± Since the P-element insertion has only one BglII site, the genomic DNA adjacent to the P- element insertion can be cloned by cutting with BglII and recovering ampR plasmids. (Enzyme indicated does not need to be BglII. Digest genomic DNA with BglII.
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This note was uploaded on 09/26/2009 for the course BIS 98659 taught by Professor Kimbrell during the Summer '09 term at UC Davis.

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Sample Midterm 2 _some 1_ Answers - Name: _ ID: _ 1. a. -...

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