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Three point mapping

# Three point mapping - Mapping with a 3 point cross Example...

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Unformatted text preview: Mapping with a 3 point cross Example F1 test cross expect VBP/vbp VBP/vbp x vbp/vbp 1 VBP/vbp : 1 vbp/vbp progeny observed genotype VBP vbp Vbp vBP VbP vBp vbP VBp number 1779 1654 252 241 131 118 13 9 4197 class parental “ single crossover “ “ “ double crossover “ Double crossovers – the one that changes from parental – tells you which gene is in the middle. In this example, P is in the middle. gene order : V – P – B (or B – P – V) Calculating map units V to P recombination events involving V single cross-over : double cross-over : Vpb (252) vPb (13) vPB (241) VpB (9) 252 + 241 + 13 + 9 x 100% = 12.3% = 12.3 map units 4197 Calculating map units single cross-over : double cross-over : P to B VPb (131) vpB (118) vPb (13) VpB (9) 131 + 118 + 13 + 9 x 100 % = 6.4% = 6.4 map units 4197 Result: V-------- 12.3 mu-------P-------6.4 mu----------B ...
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