325usum08Cancer - Cell Culture Forensics. S. Obrien PNAS...

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Unformatted text preview: Cell Culture Forensics. S. Obrien PNAS July 3, 2001 vol. 98 no. 14 7656-7658 Henrietta Lacks Cancer: Detection and Treatment Cell Culture Forensics. S. Obrien PNAS July 3, 2001 vol. 98 no. 14 7656-7658 Henrietta Lacks: the source of HeLa cells Cell Culture Forensics. S. Obrien PNAS July 3, 2001 vol. 98 no. 14 7656-7658 Henrietta Lacks: the source of HeLa cells During the 1970s and 1980s, as many as one in three cell lines deposited in cell culture repositories were imposters, one cell line overtaking or masquerading as another. The most notorious culprit was a cervical carcinoma line, HeLa, established by George Gey at the Johns Hopkins Medical School in 1951 Cell Culture Forensics. S. Obrien PNAS July 3, 2001 vol. 98 no. 14 7656-7658 Henrietta Lacks: the source of HeLa cells An estimated $10 million of research was discredited. Cell Culture Forensics. S. Obrien PNAS July 3, 2001 vol. 98 no. 14 7656-7658 Henrietta Lacks: the source of HeLa cells DNA fingerprinting has been used to differentiate different cell lines. cell lines from the same person showed 0.32.9% fragment differences HeLa cell variation QuickTime and a decompressor are needed to see this picture. J. Cell Biol. Bottomley et al. 41 (3): Fig. 3 TRENDS in Ecology and Evolution Vol 21 pg 47 Balance between Longevity and Health Mutations Cancer: is the loss of control over cell division. Tumors are normal cells that are dividing inappropriately. They stop performing their normal function, and are dividing repeatedly. A cell becomes cancerous when there are incorrect positive AND negative signals. GO! STOP! cancer Multiple mutations are required for cancer to occur Fig 22.17 Tbl 22.10 Causes of mutations: Replication errors Exacerbated by poor DNA repair Limited by telomere length Other biological agents Viruses Transposons Environmental factors Ultraviolet light Mutagenic chemicals smoking, industrial waste, natural toxins Environment plays a large role in the chance of contracting cancer The multiethnic cohort study: exploring genes, lifestyle and cancer risk. L Kolonel, D Altshuler, B Henderson (July 2004) Nature Reviews Cancer 4, 519-527 Fig 1 The rapidity of increased childhood thyroid cancer in the heavily contaminated areas of Belarus, Ukraine and Russia was surprising. 4 years Source: US Mortality Public Use Data Tape 2000, National Center for Health Statistics, Centers for Disease Control and Prevention, 2002. 1. Heart Diseases 710,760 29.6 2. Cancer 553,091 23.0 3. Cerebrovascular diseases 167,661 7.0 4. Chronic lower respiratory diseases 122,009 5.1 5. Accidents (Unintentional injuries) 97,900 4.1 6. Diabetes mellitus 69,301 2.9 7. Influenza and Pneumonia 65,313 2.7 8. Alzheimers disease 49,558 2.1 1. Nephritis 37,251 1.5 10. Septicemia 31,224 1.3 Rank Cause of Death # of deaths % of all deaths US Mortality, 2000 Change in the US Death Rates* by Cause, 1950 & 2000 * Age-adjusted to the 2000 US standard population.* Age-adjusted to the 2000 US standard population....
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This note was uploaded on 09/28/2009 for the course BIO 325 taught by Professor Saxena during the Summer '08 term at University of Texas at Austin.

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325usum08Cancer - Cell Culture Forensics. S. Obrien PNAS...

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