QB08-C - BIO320-Spring 08 Question bank C Genetic...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
BIO320-Spring 08 Question bank C 1 1997-2008 maurice kernan Genetic technology 1: restriction enzymes, DNA cloning and blot hybridization 1. The restriction enzyme BamH1 recognizes the sequence 5’GGATCC3’. What is the frequency with which this site will occur in a long random DNA sequence, with all bases present in equal proportions? A) 1/6 B) (1/6) 4 C) 1/64 D) (1/4) 6 E) 1/24 2. The restriction enzyme BamH1 recognizes and cuts the sequence 5’G/GATCC3’ in DNA. The restriction enzyme Sau3A recognizes and cuts the sequence 5’/GATC3’. (/ indicates the cut site). What fraction of Sau3A sites can be cut by BamH1, in a genome where all four bases occur with equal frequency? A) all B) ¼ C) none D) 1/16 E) ½ 3. The restriction enzyme Sal1 recognizes and cuts the sequence 5’GTCGAC3’ in DNA. The restriction enzyme HincII recognizes and cuts the sequence 5’GTYRAC3’, where R indicates either purine (A or G) and Y indicates either pyrimidine (C or T). In a genome where all four bases occur with equal frequency, what fraction of HincII sites can also be cut by SalI? A) all B) ¼ C) none D) 1/16 E) 1/2 4. A restriction enzyme recognizes and cuts the following base sequence: 5’A/CRYGT3’, where R indicates any purine (A or G), and Y indicates any pyrimidine (T or C). If this enzyme is used to cut a long, random sequence of DNA in which all 4 bases occur with equal frequency, how often would you expect the site to occur on average ? A) Once every 256 bases B) Once every 2048 bases C) Once every 512 bases D) Once every 4096 bases E) Once every 1024 bases 5. Four restriction enzymes recognize and cut the following DNA sequences (alphabetized by enzyme name; only one strand shown). R indicates either purine (A or G) and Y indicates either pyrimidine (C or T). EcoR1: 5'-GAATTC-3' HincII: 5’-GTYRAC-3’, Not1: 5’-GCGGCCGC-3’ Sau3A: 5'-GATC-3' What is the correct order for the frequency with which these enzyme sites will occur, in a long random DNA sequence with all bases present in equal amounts? (The order should be: most frequent >>> least frequent.) A. NotI > HincII > EcoR1 > Sau3A B. Sau3A > EcoR1 > HincII > Not1 C. Sau3A > HincII > EcoR1 > Not1 D. Not1 > EcoR1 > HincII > Sau3A E. HincII > Sau3A > EcoR1 > Not1 6. The subunits of a typical dimeric restriction enzyme make several types of close contact with each other and with DNA. Which of the following contacts enable a restriction enzyme to recognize a specific DNA sequence? A) Contacts between the enzyme and the bases in one strand of the DNA. B) Contacts between the two subunits of the enzyme. C) Contacts between the enzyme and the phosphates in one strand of the DNA. D) Contacts between the enzyme and the bases in both strands of the DNA.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 09/28/2009 for the course BIO 320 taught by Professor N/a during the Spring '08 term at SUNY Stony Brook.

Page1 / 14

QB08-C - BIO320-Spring 08 Question bank C Genetic...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online