BIO320 Spring 2008 Answer key for question bank C GENETIC TECHNOLOGY I: RESTRICTION ENZYMES, DNA CLONING AND BLOT HYBRIDIZATION 1. D 2. D 3. B 4. E 5. C 6. D 7. A 8. B 9. B 10. Map 4 11. A 12.D 13.A 14.D 15.A (In some versions the figure for question 13 was misplaced next to question 12). 16 ,1 restriction digest & gel electrophoresis .2 make a probe; hybridize to a Northern blot. RT-PCR can set a minimum limit to the size. .3 sequence the cloned DNA .4 Northern blot or RT-PCR, with RNA from different sources; “ in situ ” hybridization (hybridizing a probe directly to transcripts in tissue slices or whole embryos) also appropriate, but not covered yet in lecture. .5 Deduce from sequence of gene (or better, sequence of a cDNA clone) .6 Southern blot hybridization, under low-stringency conditions (to allow some mismatch between probe and similar but not identical sequences). Or PCR with degenerate oligonucleotides, based on conserved parts of the globin protein sequence. 17.B 18.D 19.A 20.C 21.B 22.B 23.B
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