Exam1-2008Key - Key_ Name (Last, First) BIO 361 _ SOLAR ID...

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Key __________________________________ _____________________________________ Name (Last, First) SOLAR ID 1 BIO 361 EXAM 1 FALL 2008 VERSION 1 NAME:______________Key______________________________ ID:_____________________________________________________ 1. Write Your name and ID on all pages 2. Write all answers in pen only 3. Make sure your exam has five pages including this cover page Page 2 _______________ Page 3 _______________ Page 4 _______________ Page 5 _______________ Total _______________
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Key __________________________________ _____________________________________ Name (Last, First) SOLAR ID 2 1-5. You have been given five samples of amino acids or peptides, but the labels have become detached from the samples. The labels are: a. Phe-Leu-Tyr b. Ser-Gly-Ser c. Hypro-Gly-Pro d. Asp e. Arg-Arg-Arg Indicate which of the labels listed could be reasonably applied to each sample described below. If none of the labels describes the sample, write NONE, and if more than one label could be applied, write the letters for each of the labels which could be applied (4 points each - points will be deducted if too many choices are given). 1. __e___ This sample is fully titrated from an initially acidic solution of below pH 2 when five equivalents of a base are added per mole of sample. 2. __c___ This sample could have been derived from tropocollagen. 3. __a,d___ This sample is fully titrated from an initially acidic solution of below pH 2 when three equivalents of a strong base are added per mole of sample. 4. __e___ This sample will emerge last from a column of sulfonated polystyrene when using a pH gradient to elute the materials. 5. __b,e___ This sample will emerge first from a gel filtration column of phenyl sepharose in which a percentage of the hydroxyl groups of highly crosslinked Sephadex™ have been modified with phenyl substituents. 6-9. You have been given a sample from a new collection of linear octapeptides (8 linked amino acids). You first attempt to cleave the peptide with cyanogen bromide. Answer the following questions TRUE or FALSE (6, 7, 8 - 3 points each; please write out TRUE or FALSE completely, not T or F. 9. 4 points) 6. __TRUE___ If you obtain a dipeptide with a C-terminal homoserine lactone, the original peptide in your sample must have had a methionine adjacent to the N-terminal amino acid. 7 __FALSE___ If you obtain a dipeptide with no homoserine lactone, the original peptide in your sample must have had a methionine directly adjacent to the C-terminal amino acid. 8. __FALSE___ If you cannot cleave your sample octapeptide with cyanogen bromide, it must not contain methionine. 9. Your sample octapeptide contains one lysine and one methionine; it is cleaved rapidly by
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This note was uploaded on 09/28/2009 for the course BIO 361 taught by Professor Lake during the Spring '08 term at SUNY Stony Brook.

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Exam1-2008Key - Key_ Name (Last, First) BIO 361 _ SOLAR ID...

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