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Unformatted text preview: BIO 361 EXAM 1 FALL 2005 NAME:_________________________________________________ ID:_____________________________________________________ 1. Write Your name and ID on all pages 2. Write all answers in pen only 3. Limit your answers to the space provided 4. Make sure your exam has seven pages including this cover page Page 2 _______________ Page 3 _______________ Page 4 _______________ Page 5 _______________ Page 6 _______________ Page 7 _______________ Total _______________ Maximum of 100 points NAME:_________________________ ID:_________________________ 2 1. Ionizable side chains. (7 points) (a) List all acidic side chains from strongest to weakest acid. (b) List all basic side chains from strongest to weakest base. 2. From the list of reagents/techniques choose the one, which is best suited for the task listed in statements (a)-(o). (15 points) 1. Urea 2. NMR spectroscopy 3. MALDI 4. Salt fractionation 5. Dialysis 6. Western blot 7. Chymotrypsin 8. Dabsyl chloride 9. t-Boc 10. Ultracentrifugation 11. Carboxymethyl group 12. DTT 13. Diethylaminoethyl group 14. DCC 15. ELISA (a) Reduces disulfide bonds (b) Determines the three-dimensional structure of proteins in solution (c) Protects the amino group of an amino acid (d) Ionizes biological macromolecules (e) Diagnostic test for HIV infection (f) Labels the N-terminal residue of a protein (g) Cleaves after aromatic residues (h) Binds anions (i) Activates carboxyl group of an amino acid (j) Allows diffusion of small, but not large molecules (k) Identifies one protein in a complex mixture...
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- Spring '08
- Biochemistry, Amino Acid Analysis, bonus points, _________________________