Exam 1-2005Key

Exam 1-2005Key - BIO 361 EXAM 1 FALL 2005

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: BIO 361 EXAM 1 FALL 2005 NAME:_________________________________________________ ID:_____________________________________________________ 1. Write Your name and ID on all pages 2. Write all answers in pen only 3. Limit your answers to the space provided 4. Make sure your exam has seven pages including this cover page Page 2 _______________ Page 3 _______________ Page 4 _______________ Page 5 _______________ Page 6 _______________ Page 7 _______________ Total _______________ Maximum of 100 points NAME:_________________________ ID:_________________________ 2 1. Ionizable side chains. (7 points) (a) List all acidic side chains from strongest to weakest acid. (b) List all basic side chains from strongest to weakest base. (a) Asp, Glu, Cys, Tyr (Asp/Glu, Cys, Tyr also correct) (b) Arg, Lys, His 2. From the list of reagents/techniques choose the one, which is best suited for the task listed in statements (a)-(o). (15 points) 1. Urea 2. NMR spectroscopy 3. MALDI 4. Salt fractionation 5. Dialysis 6. Western blot 7. Chymotrypsin 8. Dabsyl chloride 9. t-Boc 10. Ultracentrifugation 11. Carboxymethyl group 12. DTT 13. Diethylaminoethyl group 14. DCC 15. ELISA (a) Reduces disulfide bonds DTT (12) (b) Determines the three-dimensional structure of proteins in solution NMR (2) (c) Protects the amino group of an amino acid t-Boc (9) (d) Ionizes biological macromolecules MALDI (3) (e) Diagnostic test for HIV infection ELISA (15) (f) Labels the N-terminal residue of a protein Dabsyl chloride (8) (g) Cleaves after aromatic residues Chymotrypsin (7) (h) Binds anions Diethylaminoehtyl group (13) (i) Activates carboxyl group of an amino acid DCC (14) (j) Allows diffusion of small, but not large molecules Dialysis (5) (k) Identifies one protein in a complex mixture Western blo (6) (l) Separates molecules according to their size and shape Ultracentrifugation (10) (m) Unfolds proteins at high concentration Urea (1) (n) Separates proteins according to their solubility Salt fractionation (4) (o) Binds cations Carboxymethyl group (11) NAME:_________________________ ID:_________________________ 3 3. Assume that the average thickness of a biological membrane is 54 . For (a) and (b) show Assume that the average thickness of a biological membrane is 54 ....
View Full Document

Page1 / 7

Exam 1-2005Key - BIO 361 EXAM 1 FALL 2005

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online