Exam 1-2006 - _ Name (Last, First) BIO 361 _ SOLAR ID FALL...

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__________________________________ _____________________________________ Name (Last, First) SOLAR ID 1 BIO 361 EXAM 1 FALL 2006 VERSION 1 NAME:_________________________________________________ ID:_____________________________________________________ 1. Write Your name and ID on all pages 2. Write all answers in pen only 3. Make sure your exam has six pages including this cover page Page 2 _______________ Page 3 _______________ Page 4 _______________ Page 5 _______________ Page 6 _______________ Extra Credit _______________ Total _______________ Maximum of 104 points + 5 points extra credit
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__________________________________ _____________________________________ Name (Last, First) SOLAR ID 2 A. You have been given four samples, one of which is pure phenylalanine (F), one of which is pure arginine (R), one of which is pure glutamic acid (E), and one of which is a pure tripeptide, lys-trp-asp (K- W-D). You are going to perform some tests on these samples. Indicate which of the three samples will give the result described with each test (you may use the single letter codes). The possible answers include one of the samples (identify by one-letter code), more than one of the samples (indicate which), or none of the samples (if none of the samples would give the result, write NONE). __________ 1. This sample emerges first from a column of highly crosslinked dextran gel beads (Sephadex beads). ___________ 2. This sample emerges first from an anion-exchange column of DEAE-cellulose eluted with pH 8.0 buffer. ___________ 3. This sample fails to move to the anode or cathode when electrophoresed at ~pH 6.5. B. You have been given a synthetic peptide (not derived by proteolytic fragmentation of a protein) which is known to have activity against human mast cells, resulting in release of histamine. Your job is to sequence and characterize the peptide as much as possible. The amino acid composition of the peptide is: Gly 3 ,Arg 2 ,Leu 2 ,Met,Tyr, You find that reaction of the starting peptide with dansyl chloride followed by acid hydrolysis to individual amino acids yields DNS-Gly (the assay gives only qualitative information – not how much of each dansylated amino acid you obtain). You try to digest the peptide with Carboxypeptidase A, but you find that no amino acids are released. Remembering that the peptide has been synthesized, think you have an explanation for the resistance to carboxypeptidase A, but you go ahead and digest it with trypsin and react the mixture of tryptic fragments with dansyl chloride. Again, after acid hydrolysis of the mixture, you can detect DNS-Gly but no other dansylated amino acid. What bond was enzymatically cleaved with trypsin? 4. ______________ -____________ (write the amino acids with the N-terminal residue on the
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Exam 1-2006 - _ Name (Last, First) BIO 361 _ SOLAR ID FALL...

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