Unformatted text preview: would jump from 0 to -1 instead of 0 to -2. In that case, the total charge would equal 0 at pH values between the pKs for the side chain carboxyls and the imadozole side chain, i.e. 5-6. This approach ALWAYS gives you the pI – DO NOT try to figue it out by “inspection” or “intuition” and DON’T add up the pKs and average them. Remember that a peptide has only one alpha amino group and one alpha carboxyl group regardless of length, but all the ionizable side chains have pKs of their own....
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- Spring '08
- Biochemistry, pH Values, chain carboxyls