7 - MCB 102 University of California, Berkeley August 46,...

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Unformatted text preview: MCB 102 University of California, Berkeley August 46, 2009 Isabelle Philipp Handout Problem Set 7 The answer key will be posted by Friday August 7 . Try to solve the problem sets always first without the answer key. Remember, your goal is to be able to solve biochemical problems in the exams. If you can not solve one of the problems try to solve it together with one of your classmates or ask your GSI during discussion section. Dont just wait for the answer key. The answer key should only confirm that you solved the problem correctly! Exercises and Problem Sets are for extra practice and exam preparation, and should not be turned in. Recommended problems from the book: Chapter 25: 11, 12 Chapter 26: 2, 5, 6, 7, 8, 10 Chapter 27: 1, 2, 5, 9, 12, 13, 16 1. E. coli Genome Replication Assuming an error rate of 1/1000 in the primer, a primer length of 10 bps, and Okazaki fragments of 1000 bp, how many errors would you expect in the replication of the E. coli genome (4,000,000 bps) if the Okazaki fragments were primed with DNA (which is not removed from the daughter strand)? 2. Mutation Rate Suppose you wanted to make a strain of E. coli with a high mutation rate. List two genes you would mutate in order to reduce the fidelity of DNA replication. Explaingenes you would mutate in order to reduce the fidelity of DNA replication....
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This note was uploaded on 09/28/2009 for the course MCB 102 taught by Professor Staff during the Summer '08 term at University of California, Berkeley.

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7 - MCB 102 University of California, Berkeley August 46,...

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