L1 Handout - Lab 1 & Introduction

L1 Handout - Lab 1 & Introduction - Bio 1AL Lab...

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Bio 1AL Lab Lecture Outline # 1 September 1 st , 2008 I. Course Overview II. Biological Themes A. High levels of structural organization. atoms -> molecules -> macromolecules -> organelles -> cells -> tissues -> organs -> organ systems -> organism B. Making order of diversity – use of taxonomy. D > K > P > C > O > F > G > S C. Prokaryotes versus eukaryotes – presence of membrane bound organelles ("nucleus") D. Three Domains: Eubacteria, Archae, Eukarya III. Safety- common sense IV. Micropipetter A. Why – measure small volumes precisely B. Use – tips & points of resistance i) P20 – (1-20 μ L), P200 – (20-200 μ L), P1000 – (200-1000 μ L) C. Do's & Don'ts D. Sterile technique E. Exercise – weigh various volumes w/ a balance V. Microscope A. Why? – increase magnification/resolution B. Parts – imaging and illumination. C. Optical Sectioning D. Critical Illumination – condenser position, amt. of light E. Exercises 1. field of view diameter – parachute cloth 2.
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L1 Handout - Lab 1 & Introduction - Bio 1AL Lab...

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