L4 Handout - Lab 4 - Bacteria, Complementation, & Photosynthesis

L4 Handout - Lab 4 - Bacteria, Complementation, & Photosynthesis

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Bio 1AL Outline # 4 Colony Isolation, Complementation & Photosynthesis 9/22/2008 I. Enzyme lab review A. Initial velocity. B. Application of standard curves II. Cloning: Isolation, Restriction Digestion, Ligation, Transformation, Screening . A. Isolation and culturing of transformed E. coli . B. Screening- use of genetic markers/traits of pBLU C. Use of controls. Positive and negative. III. Complementation A. Main concepts i) Ploidy ii) Yeast life cycles and mating types: ( a and ! ) iii) Histidine biosynthesis pathway iv) Mutations v) Experimental design using YPD & SD plates IV. Photosynthesis – focus on light dependent reactions. A. 6 CO 2 + 12 H 2 0 - ------- -> C 6 H 12 O 6 + 6 H 2 0 + 6 O 2 B. Lots of intermediates – pigment:protein complexes capture light, many enzymes, e - carriers, proton gradient, oxidation/reduction reactions, ATP synthase, etc. C. Two major aspects: Light dependent reactions & Light independent (Calvin cycle) D. Where? In eukaryotes in chloroplast. i) leaf structure
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 09/28/2009 for the course BIO 1al taught by Professor Pederson during the Fall '08 term at Berkeley.

Page1 / 2

L4 Handout - Lab 4 - Bacteria, Complementation, & Photosynthesis

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online