L4 Handout - Lab 4 - Bacteria, Complementation, & Photosynthesis

L4 Handout - Lab 4 - Bacteria, Complementation, & Photosynthesis

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Bio 1AL Outline # 4 Colony Isolation, Complementation & Photosynthesis 9/22/2008 I. Enzyme lab review A. Initial velocity. B. Application of standard curves II. Cloning: Isolation, Restriction Digestion, Ligation, Transformation, Screening . A. Isolation and culturing of transformed E. coli . B. Screening- use of genetic markers/traits of pBLU C. Use of controls. Positive and negative. III. Complementation A. Main concepts i) Ploidy ii) Yeast life cycles and mating types: ( a and ! ) iii) Histidine biosynthesis pathway iv) Mutations v) Experimental design using YPD & SD plates IV. Photosynthesis – focus on light dependent reactions. A. 6 CO 2 + 12 H 2 0 - ------- -> C 6 H 12 O 6 + 6 H 2 0 + 6 O 2 B. Lots of intermediates – pigment:protein complexes capture light, many enzymes, e - carriers, proton gradient, oxidation/reduction reactions, ATP synthase, etc. C. Two major aspects: Light dependent reactions & Light independent (Calvin cycle) D. Where? In eukaryotes in chloroplast.
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