L6 Handout - Lab 6 - GMB II

L6 Handout Lab 6 - Bio 1A Lab Lecture Outline 6 GMB II Oct 6th 2008 I Warning Lab lecture does not replace reading the lab manual and reading

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Bio 1A Lab Lecture Outline # 6 GMB II Oct. 6 th , 2008 I. Warning: Lab lecture does not replace reading the lab manual and reading Campbell. A. Practice problems. B. Practice lab exams Lab Exam Reader. Do them, expect somewhat similar problems. II. Electrophoresis (loading on p. 83) A. Overview of DNA migration in agarose gel. B. Migration dependent upon size and conformation C. Linear: smaller pieces migrate faster than larger pieces. D. Conformation: all three conformations are the exact same size. 1) Super coiled migrates fastest. 2) Linear migrates intermediate. 3) Relaxed (or nicked) migrates the slowest. E. Conformation definitions: converting supercoiled to relaxed to linear. Surface of Buffer (1X TBE) λ - λ B 1 2 3 4 5 6 7 8 9 10 λ E Figure 1: Loading the gel. Note the order of loading of the samples. Lanes 1 and 10 get diluted loading dye. Lanes 2-5 get various forms of λ DNA. Lanes
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 09/28/2009 for the course BIO 1al taught by Professor Pederson during the Fall '08 term at University of California, Berkeley.

Page1 / 3

L6 Handout Lab 6 - Bio 1A Lab Lecture Outline 6 GMB II Oct 6th 2008 I Warning Lab lecture does not replace reading the lab manual and reading

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online