Review Session Exam 1 Oct 10

Review Session Exam 1 Oct 10 - Controls Why What would they...

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Controls • Why? • What would they include and why?
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Resolution: Agarose gel Standard curve Distance migrated (mm) # of bp (semi-log) ! DNA Hind III digest Detection methods - EtBr, radioactivity-- # of molecules.
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+ - Migration is dependent upon shape! L R SC Detection methods - EtBr, radioactivity-- # of molecules.
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1N, 1C 1N, 1C 2N, 2C 2N, 4C Campbell 13.5 Campbell 13.3 Ploidy -sets of chromosomes (N vs. C)
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Genes & location (locus) & mutation Loss of function Mutants: single mutation, recessive Info from Mom Info from Dad
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Complementation: Key concepts Yeast life cycle-1N & 2N Mitosis Mitosis
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a’ b’ c’ d’ e’ f’ g’ h’ Biosynthetic pathways Analysis in a diploid. d’ f’ Analysis in a haploid. 2 sets 1 set
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a’ b’ c’ d e’ f’ g’ h’ Histidine (A.A). Remember this enzyme is encoded by a gene. Defective enzyme = mutant gene. Rescue by the addition of a chemical? Rescue by the addition of a good gene? - Mating CHEMICALS DO NOT RESCUE Complementation: Pathway CHEMICALS COULD RESCUE
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Complementation: Key concepts Mating- generating diploids a’ b’ e’ f’ g’ h’ Histidine (A.A). c’ d’ K1 a’ b’ e’ f’ g’ h’ Histidine (A.A). c’ d’ M1 a’ b’ e’ f’ g’ h’ Histidine (A.A). c’ d’ K1 a’ b’ e’ f’ g’ h’ Histidine (A.A). c’ d’ M2 YPD plate SD plate NO Histidine Replica plate Time: Growth? Cross 1 Cross 2
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Complementation i) Scoring growth! ii) # of complementation groups! iii) Order of the pathway? Chemical accumulation & rescue. a’ b’ e’ f’ g’ h’ Histidine (A.A). c’ d’ M2 Growth? YPD plate SD plate
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1 2 3 4 5 6 7 Accumulates Rescued by 1 - - + + - + + C A, B, D 2 - + + - + + C A, B, D 3 - + + + - A B, D 4 - + + + E A, B, C, D 5 - + + C A, B, D 6 - + D B 7 - A Least # of genes? Order of path? Complementation groups? Yes, there can be only one member of a group. What accumulates and what rescues?
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Mendelian Genetics 1) Meiosis!!!!!!!!!!!!!!!!!!!!!!! 2) N vs C 3) Notation - reflects alleles on chromosomes.
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Review Session Exam 1 Oct 10 - Controls Why What would they...

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