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Lab 4 - Picking a colony Complementation Photosynthesis...

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A21 Picking a colony, Complementation & Photosynthesis Pre-lab . Name GSI & Sect # Station # To answer these questions read all of the lab material on constructing recombinant DNA (pages 60-86) even though you are only doing pages 73 & 74 for lab 4. You also need to read about complementation (pages 107- 114) even though you are only doing pages 107-109. You also need to read about photosynthesis on pages 115- 129. Write legibly. You will NOT be admitted to lab unless you turn in this completed pre-lab at the start! 1. What color of colony should you select on your LB/AMP/X-gal plate if you had an insert? What color of colony should you select on your LB/AMP/X-gal plate if you had no insert? 2. What is histidine? What is the ploidy of the mutant yeast cells that you are using in the complementation experiment? Are they auxotrophs, or prototrophs? Explain your answer. 3. In your own words, very briefly describe how one performs pair-wise mating of yeast strains.
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