Lab 5 - Plasmid isolation and digestion, Complementation...

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A25 Plasmid isolation and digestion, Complementation II, and GMB I Pre-lab. Due at the start of lab. N a m e GSI & Sect # Station # To answer these questions read all of the lab material on constructing recombinant DNA (pages 60-86) even though you are only doing pages 75-80 for lab 5. You also need to read about complementation (pages 107-114) even though you are only doing pages 110-112. You also need to read about GMB I (pages 131-140). Write legibly. You will NOT be admitted to lab unless you turn in this completed pre-lab at the start! 1) Define the following terms: binding affinity: crossing over: homologous chromosomes: complementation: restriction enzyme: 2) Can yeast, deficient in histidine biosynthesis, grow on YPD media? on SD media? 3) Where do you dispose of the used pipette tips and used centrifuge tubes? 4) Discuss the DNA binding affinity of the spin column and how its affinity changes with changes in ionic concentration. 5)
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This note was uploaded on 09/28/2009 for the course BIO 1al taught by Professor Pederson during the Fall '08 term at University of California, Berkeley.

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Lab 5 - Plasmid isolation and digestion, Complementation...

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