Lab 3 - Transformation and Enzyme Worksheet. Due at the...

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A17 Transformation and Enzyme Worksheet. Due at the start of your next lab. N a m e GSI & Sect # Station # 1. Did you label the bottom of both of your plates and then tape the two plates together using small pieces of tape? Circle one: Yes / No Did you place them in the tray upside down (agar up) at the instructors bench? Yes/ No 2. a) Graph your results from Part I- Relationship between enzyme concentration and enzyme activity . You must convert Optical Density into µmoles of maltose. 0 100 200 300 400 2. b) Would you expect a linear or non-linear dependence based upon how enzymes function as catalysts? Explain in terms of a simple model for enzyme catalysis. See the tutorial if needed.
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A18 3. a) Graph the results of one set of data for part II-A. DO NOT AVERAGE CLASS DATA – instead pick the best set of data (or plot your own if you did this part). 0 25 50 75 100 3. b) What is the temperature optimum of salivary amylase? How does this compare to body temperature? (include units) 4. Explain the basis for a temperature optimum in terms of enzyme structure and kinetics.
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This note was uploaded on 09/28/2009 for the course BIO 1al taught by Professor Pederson during the Fall '08 term at University of California, Berkeley.

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Lab 3 - Transformation and Enzyme Worksheet. Due at the...

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