Lab 4 - Photosynthesis Worksheet. Due at the start of your...

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A23 Photosynthesis Worksheet. Due at the start of your next lab. N a m e GSI & Sect # Station # 1. Present the results of your four experiments with chloroplast extract in one graph. Plot the absorbance of DCPIP against time. 0 75 150 225 300 2. a. Calculate the rate of DCPIP reduction (= rate of absorbance change) for methyl amine, DCMU, and the light control (use only the decreasing part of the curve for your calculations). Include units. i) RATE LIGHT ii) RATE METHYL AMINE iii) RATE DCMU b. What is the mechanism underlying DCMU? What is the mechanism underlying methyl amine? 3. What is the purpose of the dark control? What is the purpose of the light control? (What are you checking in both cases?) Explain. Worksheet Continues Time (seconds)
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A24 4. Indicate your results for the chromatography. Include the R f values for all 3 carotenoid bands and for Chlorophyll a and Chlorophyll b pencil line direction of solvent migration Label solvent front, pigment location, pigment name, color and identity. Include Rf values by each.
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Lab 4 - Photosynthesis Worksheet. Due at the start of your...

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