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Lab _2 - Chol - Measurement of Cholesterol in Biological...

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Measurement of Cholesterol in  Biological Samples Objectives: to develop a basic understanding of cholesterol, lipoproteins, HDL and LDL. to quantitate total cholesterol , HDL and LDL levels in various serum samples. to learn the “chemistry” behind clinical tests.
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functional importance: the precursor of bile acids, steroid hormones and vitamin D. structural importance: a major component of animal plasma membrane. 60-70% cholesterol in blood is esterified. Ester Cholesterol
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Cholesterol is transported as a complex with  lipoprotein http://www.uwsp.edu/chemistry/tzamis/ch260/lipoprotein.jpg
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Chylomicrons – largest proportion of lipids Very low-density lipoproteins (VLDL) Low-density lipoproteins (LDL) -“bad cholesterol”; transports cholesterol from liver to tissues. High-density lipoproteins (HDL) – least proportion of lipids, “good cholesterol”; transports cholesterol from tissues to liver  Lipoproteins are classified by density and  composition.  High LDL:HDL ratio indicates higher incidence of heart disease.
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  Atherosclerosis  is a chronic cardiovascular  disease as result of plaque formation in the artery http://www.heart-health-for-life.com/cholesterol-and-heart-disease.html LDL is associated with depositing cholesterol in arterial plaques HDL appears to reduce cholesterol deposit Atherosclerosis hypertension heart attack and stroke Measurement of cholesterol is a routine clinical test.
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