Lab _3 - Affinity Chromatography

Lab _3 - Affinity Chromatography - Affinity Chromatography...

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To understand the principle of affinity chromatography To purify enzyme trypsin from a contaminating protein, bovine serum albumin (BSA). To confirm presence of functional trypsin by conducting trypsin activity assay. Affinity Chromatography Purification
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Tubing Sample Column Stopcock Chromatography set up What is chromatography ? A physical method used to separate complex mixtures. Two-phase process (stationary and mobile). Different types of chromatography: Thin-layer Chromatography (TLC) High-performance Liquid Chroma. (HPLC) Ion-exchange Size-exclusion Affinity – today’s lab
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affinity chromatography ? a type of chromatography based on natural specificity (interaction) of biological molecules. Enzyme substrate analogue, inhibitor, cofactor. Antibody antigen, virus, cell. Nucleic acid complementary base sequence, nucleic acid polymerase, and nucleic acid binding protein, e.g . poly T for poly A mRNA purification. Hormone, vitamin
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This note was uploaded on 09/28/2009 for the course EXSC 205 taught by Professor Girandola during the Spring '08 term at USC.

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Lab _3 - Affinity Chromatography - Affinity Chromatography...

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