Lab _5 - Beta-Gal (ion-exch)

Lab _5 - Beta-Gal (ion-exch) - Next three lab sessions:...

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Unformatted text preview: Next three lab sessions: formal lab report labs 1 st Lab : salting out of -gal and determine ideal - gal concentration range to work with. 2 nd Lab : Purification of -gal using ion- exchange chromatography. rd Purification & Characterization of E. coli - galactosidase ( -gal) Objectives to complete salting-out procedure from last week. to further purify galactosidase using ion-exchange chromatography. Purification of -Gal using Ion- exchange Chromatography Overview of Todays Experiment (NH 4 ) 2 SO 4 precipitated sample 17, 000 RPM Supernatant Pellet ( -gal) -Gal and Bradford Assays (no dilutions) Resuspend in 3.5 ml Z-Buffer 0.5 ml 3.0 ml Ion-Exchange Chrom. -Gal and Bradford Assays (dilute sample) Ion Exchange Chromatography http://www.waters.com/webassets/cms/category/media/other_images/primer_T_Ion_Exchange_CHromatography.jpg Principle of Ion Exchange Chromatography...
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Lab _5 - Beta-Gal (ion-exch) - Next three lab sessions:...

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