Lab _6 kinetics lab

Lab _6 kinetics lab - MichaelisMenten Enzyme Kinetics Part...

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Michaelis-Menten Enzyme Kinetics
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Part A.  To study the enzymatic kinetics of  β - galactosidase.  Part B.  To examine the effects of a competitive and a  non-competitive inhibitor on the activity of   β - galactosidase –  reactions in the presence of lactose and  EDTA.
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What is “enzyme kinetics”? Study of the reaction rates of chemical reactions that are  catalyzed by enzymes. How rates differ by… Substrate concentration Enzyme concentration Inhibitors Michaelis-Menten Model of a  single substrate reaction
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Saturation curve for an enzyme showing the relation  between the concentration of substrate and rate.
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Double reciprocal plot of 1/V versus 1/[S] allowing one  to derive K m  and V max  values from rates of catalysis at  different [S] 
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Competitive Non-Competitive
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Competitive Non-Competitive http://www.steve.gb.com/images/science/ with inhibitor no inhibitor with inhibitor no inhibitor
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Competitive Non-Competitive
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(1/100 or 1/200)
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Lab _6 kinetics lab - MichaelisMenten Enzyme Kinetics Part...

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