LAB 4 Purification B-Gal

LAB 4 Purification B-Gal - (ml) 1.3 1.3 1.3 1.3 1.3 1.3 1.3...

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Results and Analysis: Table 1: Setting up Serial Dilutions Dilutio n 1:5 1:10 1:20 1:50 1:100 1:200 1:400 1:800 1:1000 Cell Extrac t 0.8 2.0 ml of 1:5 2.0 1.6 2.0 2.0 2.0 2.0 3.2 Z Buffer 3.2 2.0 2.0 2.4 2.0 2.0 2.0 2.0 0.8 Total Volum e 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 Table 2: Bradford Assay Tube # 1 2 3 4 5 6 7 8 9 10
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Dilutio n Blan k 1:5 1:10 1:20 1:50 1:100 1:200 1:400 1:800 1:1 000 Diluted Cell Extract (ml) 0.1 buffe r 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Bradfor d Reagen t (ml) 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Total Volume (ml) 3.1 3.1 3.1 3.1 3.1 3.1 3.1 3.1 3.1 3.1 A 595 0 0.471 0.30 5 0.15 0 0.119 0.050 0.055 0.049 0.105 0.0 24 Table 3: β -Gal Assay Tube # 1 2 3 4 5 6 7 8 9 10 Dilutio n Blan k 1:5 1:10 1:20 1:50 1:100 1:200 1:400 1:800 1:1000
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Diluted Cell Extract (ml) 0.2 buffe r 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Z Buffer
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Unformatted text preview: (ml) 1.3 1.3 1.3 1.3 1.3 1.3 1.3 1.3 1.3 1.3 ONPG (ml) 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Total Volume (ml) 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 A 420 >3 >3 2.00 7 1.28 1 0.487 0.112 0.030 0.025 0.010 Data Table: Cell Extract before Purification Volume (ml) Standard Dilution (Bradford Assay) Abs 595 nm Standard Dilution ( -Gal Assay) Abs 420 nm Activity ( mole/ml * min) Protein (mg/ml) Total Activity ( mole/min) Total Protein (mg) Protein Yield (%) 100 Enzyme Yield (%) 100 Specific Activity ( mole/min * mg)...
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This note was uploaded on 09/28/2009 for the course EXSC 205 taught by Professor Girandola during the Spring '08 term at USC.

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LAB 4 Purification B-Gal - (ml) 1.3 1.3 1.3 1.3 1.3 1.3 1.3...

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