4_10_07 - April 10, 2007 Biology 130 Protein Synthesis One...

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April 10, 2007 Biology 130 Protein Synthesis One of most important parts of biology Gene o Informational unit which codes for polypeptide chains o One gene one enzyme hypothesis How to figure out metabolic pathways and the genes required for them o How is this information used First step: Transcription Production of mRNA – blueprint mRNA combines specific amino acids in peptide bonds to form a polypeptide Prokaryotic o almost simultaneous reaction of mRNA and polypeptide formation eukaryotic o edit pre-mRNA with splicing to make mRNA o genetic engineering is much more complex codon o amino acid consisting of three letters of mRNA (triplet code) o on mRNA always starts at methionine AUG always stops at UAA, UAG, UGA almost only need two letters for coding Wobble experiment – often mutation in third letter doesn’t have too much impact in coding Unravel DNA to access for transcription Binding of transcription factors (eukaryotes only) o Make the promoter accessible for RNA polymerase to bind o Always promoter and terminator TATA box – start of promoter RNA polymerase II is for polypeptide RNA RNA polymerase I is for ribosomal RNA
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This note was uploaded on 09/29/2009 for the course NPB NPB 101 taught by Professor Weidner/wingfield during the Spring '08 term at UC Davis.

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4_10_07 - April 10, 2007 Biology 130 Protein Synthesis One...

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