Ashkan Sajedi mcim 417 assignment CRISPR.docx - Ashkan Sajedi 11225055 Dr Joyce Wilson MCIM 417 CRISPR Assignment Q1 a Application of CRISPR gene

Ashkan Sajedi mcim 417 assignment CRISPR.docx - Ashkan...

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Ashkan Sajedi Dr. Joyce Wilson 11225055 MCIM 417 CRISPR Assignment Q1 a) Application of CRISPR gene editing in treatment of a genetic disease. CRISPR-Cas9 is a tool which lets us edit any genetic mutation at will, allowing us to cure the disease it causes. Cystic Fibrosis is a genetic disease that causes severe respiratory problems, and by using the CRISPR technology we could be able edit the mutations that cause Cystic Fibrosis by editing the mutations that are located in a gene called CFTR. The CRISPR technology can also be used in screening, by modifying thousands of genes to assess their function in a single experiment. Any essential genes that are modified could lead to development of a therapeutic drug. Q1 b) CRISPR screening uses this technology to enable thousands of genes to be modified and their functions be assessed in a single experiment. The results obtained from the screen can help identify what essential genes are required for the invading virus to infect and replicate.
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