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Genetics Exam I Notes

Genetics Exam I Notes - Semi-conservative Parental DNA...

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Griffith- Heat-killed bacteria (R strain) and virulent bacteria (S strain) Avery- Transforming principle; extracts were mixed with living bacteria, incubated, and plated on petri dish; either polysaccharides, proteins, DNA, or RNA were responsible for the transformation of R strain to S strain. Hershey and Chase- Phage T2; DNA or RNA was the genetic material Mesleson and Stahl- DNA replication at the molecular level; light and heavy isotopes of nitrogen were used. Conservative- Two parental DNA strands remain together during replication so that one progeny contains both parental and the other only newly synthesized DNA
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Unformatted text preview: Semi-conservative- Parental DNA strands separate and serve as templates Dispersive- Parental double helix is broken into double-stranded DNA segments Beadle and Tatum- One gene one enzyme hypothesis which states that there is a specific relationship between genes and enzymes; each gene controls the synthesis/activity of an enzyme.- Wild-type neurospora- Can survive in minimal media containing a carbon source, biotin, and inorganic salts Topoisomerase I- Positive supercoil; brings back to relaxed state Topoisomerase II- Negative supercoil; compacts DNA...
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