10 - 1 Lecture 10 October 8, 2008 Aggrecan, Perlecan and...

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Unformatted text preview: 1 Lecture 10 October 8, 2008 Aggrecan, Perlecan and chondrodysplasia 2 (8) Oct 1 Transmembrane and FACIT collagens: skin blistering and cartilage defects Proteoglycans (9) Oct 6 Hyaruronan, aggrecan and chondrogenesis (10) Oct 8 Aggrecan and cartilage defects Perlecan: endochondral ossification and chondrodysplasia Oct 13 Thanksgiving University closed Oct 15 Term Test #1: collagens (11) Oct 20 Small leucine-rich proteoglycans (SLRPs): Decorin and biglycan and osteogenesis (12) Oct 22 Transmembrane proteoglycans: syndecan and growth factor signaling (13) Oct 27 Integrins and cell-matrix adhesion (14) Oct 29 Matrix remodeling metalloproteinases, ECM remodeling and cancer 3 Hyaluronan Aggrecan Keratan sulfates (KS) Chondroitin sulfates (CS) Non-covalent interactions between HA and the G1 domain of aggrecan and link protein (LP). LP G2 G1 Link protein shares sequence and structural similarity to G1 NH 2 COOH LP G1 Non-covalent interaction with hyaluronan 4 Core protein GAG An aggrecan aggregate from fetal bovine cartilage An electron micrograph of an aggregate shadowed with platinum Numerous free aggrecan molecules are visible Approx. 100 aggrecan monomers non-covalently bound to a single HA chain through 2 link proteins that bind the core protein of the PG and HA chain. MW>10 8 ~ the size of a bacterium Aggrecan aggregate 5 Proteoglycans in the extracellular matrix of rat cartilage Aggrecan Core protein Type II collagen fibril Freeze substitution technology (rapid freezing at -196 o C) 6 Hay E (1991) Cell Biol of ECM 2 nd Ed Plenum Fig. 2.6Ed Plenum Fig....
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10 - 1 Lecture 10 October 8, 2008 Aggrecan, Perlecan and...

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