12 - 1 Lecture 12 October 22, 2008 Proteoglycans Biglycan...

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Unformatted text preview: 1 Lecture 12 October 22, 2008 Proteoglycans Biglycan and osteogenesis The syndecan family of transmembrane PGs and cell-matrix adhesion: synergistic signaling with integrins 2 Final Exam Wednesday, December 12 9-11 a.m. Upper Small Gymnasium Third Floor Benson Building 320 Huron Street 3 (8) Oct 1 Transmembrane and FACIT collagens: skin blistering and cartilage defects Proteoglycans (9) Oct 6 Hyaruronan, aggrecan and chondrogenesis (10) Oct 8 Aggrecan and cartilage defects Perlecan: endochondral ossification and chondrodysplasia Oct 13 Thanksgiving University closed Oct 15 Term Test #1: collagens (11) Oct 20 Small leucine-rich proteoglycans (SLRPs): Decorin and collagen fibrilogenesis (12) Oct 22 Biglycan and osteogenesis Transmembrane proteoglycans: syndecan and growth factor signaling (13) Oct 27 Integrins and cell-matrix adhesion (14) Oct 29 Matrix remodeling metalloproteinases, ECM remodeling and cancer 4 Dr. Jaro Sodek, Matrix Dynamics Groups, Faculty of Dentistry, UofT Bone Proteoglycans Focus of the lecture Biglycan (CS-PG1) 5 Dr. Jaro Sodek, Matrix Dynamics Groups, Faculty of Dentistry, UofT Small Small Small calcium binding glycoproteins enriched in mineralized tissues SPARC/osteonectin/BM40 SPARC/osteonectin/BM40 Osteocalcin Bone sialoprotein Osteopontin Osteopontin HA =Hydroxyapatite 6 Osteoclast Cement Layer Osteoid Lining Cells OPN Mineralized Bone Matrix Proteins in Bone Formation HP-OPN BSP OC OPN BSP HP-OPN Differentiation COL I ALP COL I Dr. Jaro Sodek, Matrix Dynamics Groups, Faculty of Dentistry, UofT Osteoblasts Pre-osteoblasts Osteocyte Biglycan: involved in the rowth and differentiation of pre-osteoblasts cells 7 A defect in the differentiation of biglycan-KO osteoblastic cells Chen et al (2004) FASEB J. 18:948-958 Expression of bone proteins in an in vitro culture system of calvarial osteoblastic cells pn- osteopontin sp- bone sialoprotein c- osteocalcin Calvarial osteoblastic progenitors are derived from mesenchymal cells- intramembranous osteogenesis Days in culture Northern Blot (RNA) No difference in type I collagen, decorin and fibromodulin RNA expression between wt and KO cells opn 8 Chen et al (2004) FASEB J. 18:948-958 Ca 2+ accumulation demonstrated by Alizarin red straining in neonatal calvarial cell cultures Bgn-KO Wild-type Cells cultured for 4 weeks postconfluence in the presence or absence of Bone Morphogenic Protein-4 (BMP-4) Duplicate wells BMP-4, a growth actor that stimulates...
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12 - 1 Lecture 12 October 22, 2008 Proteoglycans Biglycan...

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