BIOL 215 Exam 1 Review Slides 2007

BIOL 215 Exam 1 Review Slides 2007 - KaiserImam...

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Kaiser Imam [email protected] 1
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vmax and Keq The velocity equation for v max  includes the  concentration of enzyme (actually, the  concentration of enzyme  active sites ):   v max  = k 2 [E] = k cat [E] K m  is a substrate concentration and is  independent of the concentration of  enzyme. 2
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Question 5 The plot in b) would be expected to be  obtained.   If the total amount of enzyme ([E]) is  increased, v max  will increase since v max  =  k cat [E].   Remember that K m  is unaffected by  changes in enzyme concentration.  Look for plots that show changes in v max   without changes in K m 6
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Competitive Inhibition Inhibitor  and  substrate  compete  for  binding  directly to the active site Inhibition  can  be  overcome  by  adding  more  substrate If  [S]  is  high  enough,  the  substrate  can  outcompete  the  inhibitor,  reaching  the  original  v max In competitive inhibition  v max  is the same  for an  enzyme with and without an inhibitor  8
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Uncompetitive Inhibition Inhibitor binds to an allosteric site, making it  more difficult for the substrate to bind to the  active site Inhibitor cannot be displaced by adding  substrate, no matter how much substrate you  add K m  is reduced and v max  decreases v max /K m  is the same  for the enzyme with and  without the inhibitor present 9
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Noncompetitive Inhibition Also called mixed inhibition Inhibitor binds to an allosteric site Unlike in uncompetitive inhibition, a  noncompetitive inhibitor can bind the  enzyme with or without the binding of the  substrate v max  is reduced K m  is the same  for the enzyme with and  without an inhibitor 10
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11 Michaelis-Menten with Enzyme  Inhibition E  +  S      ES        E  +   P                 +                   + I                    I                 ↓↑                ↓↑ EI                ESI 1 2
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Competitive Inhibition Taken from “Principles of Biochemistry” 3rd Edition, 2002, Horton, et al. Prentice Hall 12
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Taken from “Principles of Biochemistry” 3rd Edition, 2002, Horton, et al. Prentice Hall 13
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Taken from “Principles of Biochemistry” 3rd Edition, 2002, Horton, et al. Prentice Hall 14
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16 Uncompetitive Competitive Noncompetitive
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This note was uploaded on 09/29/2009 for the course BIO 215 taught by Professor Diiulio during the Spring '09 term at Case Western.

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BIOL 215 Exam 1 Review Slides 2007 - KaiserImam...

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