9-14-09 - beta catenin activates lhx3 Lhx3 is necessary and...

Info iconThis preview shows pages 1–12. Sign up to view the full content.

View Full Document Right Arrow Icon
Bilateral symmetry in the tunicate
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Fate mapping in the tunicate
Background image of page 2
Cytoplasmic rearrangement in the tunicate Yellow & clear cytoplasm stream to vegetal pole following sperm entry
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Yellow crescent formation dependent on microtubules and the Ca ++ wave
Background image of page 4
for muscle development
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Macho-1 mRNA localizes in yellow cytoplasm containing cells LOF (antisense oligos) & GOF experiments demonstrate macho-1 is necessary and sufficient for yellow cytoplasm muscle macho-1 activates tbx6 (necessary for muscle) and snail (inhibits brachyury necessary for notochord)
Background image of page 6
β -catenin protein localizes to future endoderm endoderm is from A4.1 & B4.1 b catenin is necessary & sufficient for endoderm
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Subtraction screen IDs lhx3 as a β catenin target GOF LOF (Satou, Development 2001)
Background image of page 8
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 10
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 12
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: beta catenin activates lhx3 Lhx3 is necessary and sufficient for endoderm LOF: morpholino GOF: mRNA normal normal (Satou, Development 2001) b cat->lhx3-> endoderm The two-step process for specifying the marginal cells of the tunicate embryo A4.1 derivatives B4.1 derivatives 2 roles of Fgf: promote notochord, inhibit muscle brachyury tbx6 & snail Autonomous specification in tunicates Toolbox • Localization: Antibodies and in situ hybridization • Loss-of-function: antisense oligos • Gain-of-function: mRNA injection • Fate mapping in specification: genetic (transplant), ablation, fluorescent labeling. • Commitment: isolation, recombination (transplant) • Transgenics • Subtraction screen...
View Full Document

This note was uploaded on 09/29/2009 for the course BIO 349 taught by Professor Wallingford during the Spring '09 term at University of Texas.

Page1 / 12

9-14-09 - beta catenin activates lhx3 Lhx3 is necessary and...

This preview shows document pages 1 - 12. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online