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Introduction Biol5la

Introduction Biol5la - ArthurNguyen Introduction Lab 5...

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Arthur Nguyen Introduction Lab 5 Question #1 How will increasing substrate concentration affect the rate (vo) an enzyme reaction if enzyme concentration is held constant for different trials? Hypothesis The enzyme concentration is held at constant and substrate concentration is increase which cause the rate (vo) an enzymatic reaction to increase Background information Enzymes are protein, which have a unique conformation, increase chemical reactions because it lowers the activation energy. Since enzyme is a catalytic protein, it is a catalyst, which is a chemical agent, speeds up reaction without being consumed by the reaction (Campbell “biology” 7 th , pp. 150). Substrate binds to active site, which has a specific-pocket shape. When substrate binds to active site, the quaternary shape of enzymes will change and will create an induce fit easily bind them together. The active site act as a template for substrate orientation, stabilizes the transition states, and participates with catalytic reaction. During catalytic reaction, molecule of substrate will look for available active site and binds to enzyme. The enzymes convert the substrate to product and products are release (Campbell “Biology” 7 th , pp153). Also, the amino acids have side chains (R) to make up the active site and convert substrate to product, which then depart from the active site (Campbell “Biology” 7 th , pp 152). Because enzymes can regulate the rate of reaction without being chemically changed, it can catalyze an infinite number of conversions of substrate to products (Bio 05LA Lab Manual-lab #5, UCR, winter ’09 ed., pp.
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