HW 2 - HW#2 Due: Monday, Sept 28 (at 9:00pm) 1. In order to...

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HW#2 Due: Monday, Sept 28 (at 9:00pm) 1. In order to measure the enzyme activity and the initial rate of reaction, 5 ml of cellobiose (10 μ mol/ml) and 44 ml of buffer solution were placed in a stirred vessel. The reaction was initiated by adding 1 ml of enzyme ( β -glucosidase) solution which contained 0.1 mg of protein per ml. At 1, 5, 10, 15, and 30 minutes, 0.1 ml of sample was removed from the reaction mixture and its glucose content was measured. The results were as follows: Time (min) Glucose concentration ( μ mol/ml) 1 0.05 5 0.23 10 0.38 15 0.52 30 1.03 a. What is the activity of the β -glucosidase in units/ml of enzyme solution and in units/mg protein? A unit is defined as the enzyme activity which can produce 1 μ mol of product per minute. b. What is the initial rate of reaction? 2. Consider the following reaction sequence: Develop a suitable rate expression for product-formation [ v = k 5 (ES) 2 ] by using (a) the rapid equilibrium approach, and (b) the quasi-steady-state approach.
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HW 2 - HW#2 Due: Monday, Sept 28 (at 9:00pm) 1. In order to...

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