Lecture Note 4

Lecture Note 4 - Enzyme Reactor with simple kinetics(reference book Biochemical Engineering by James M.Lee Bioreactor-a device within which

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1 1 Enzyme Reactor with simple kinetics ( reference book: Biochemical Engineering, by James M.Lee ) •B io reac to r -a device within which biochemical transformation are caused by the action of enzymes or living cells. • Bioreactor is frequently called a fermenter, but we call bioreactor employing enzymes an enzyme reactor to distinguish it from the bioreactor which employs living cells, the fermenter.
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2 2 V V F [S] 0 F [S] f t=0 t [S] Batch Reactor Continuous Stirred-Tank Reactor (CSTR) Plug-Flow Reactor (PFR)
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3 3 Batch Reactor • No flow-in and flow-out • Equipped with an agitator to mix the reactants • The pH is maintained by employing either a buffer solution or a pH controller. • An idea batch reactor is assumed to be well mixed so that the contents are uniform in composition at all times V, [S] [S]=[S] 0 at t=0
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4 4 Batch Reactor Substrate Mass Balance: Accumulation=flow in –flow out + generation V, [S] [S]=[S] 0 at t=0 m K S S v dt S d v + = = ] [ ] [ ] [ max v dt V N d v Vdt dN V N S V v N v V dt dN S S S S S = => = => = = ] [ lume reactor vo rate reaction enzymatic t at time substrate of Moles Like enzyme batch kinetics
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5 5 Batch Reactor V, [S] [S]=[S] 0 at t=0 m K S S v dt S d v + = = ] [ ] [ ] [ max ] [ ] [ ln ] [ ] [ or ] [ ] [ ln ] [ ] [ 0 0 max 0 0 max S S t K t S S v S S K S S t v m m = + = predicted. be can reactor batch a in with time ] [ of change the , and of es known valu With reactor. batch in time respect to with changing is [S] how shows equation This max S K v m yield n to integratio by
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6 6 Continuous Stirred-Tank Reactor (CSTR) • CSTR is an ideal reactor which is based on the assumption that the reactor contents are well mixed .
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This note was uploaded on 10/01/2009 for the course CHEM 334 taught by Professor Lei during the Spring '09 term at UConn.

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Lecture Note 4 - Enzyme Reactor with simple kinetics(reference book Biochemical Engineering by James M.Lee Bioreactor-a device within which

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