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PRACTICAL REPORTMarkFaculty:ScienceCourse:Biomedical ScienceUnit Code & Title:UDDD2234 MicrobiologyTrimester:May 2018Title:Experiment 4: Microbial ControlPractical Group:P1 (Tue 12-2 pm)P2 (Tue 3-5 pm)P3 (Wed 12-2 pm)P4 (Thu 9-11 pm)Date of Submission: 6.8.2018Instructor:Ms. Teo Kah ChengDeclaration:We certify that this report is our own work, where material and reference used from othersources have been properly acknowledged.Group membersNameID NumberSignature1AW WEI WEI16ADB031062JEFFREY LING SHU HAO17ADB022873TAN YEU LUN16ADB026664THEY HUI WEN16ADB026791dddd
TITLE:Isolation of soil bacteriaOBJECTIVES:1.To compare the effect of ultraviolet (UV of 254 nm) radiation under different durationbetween different types of microorganisms.2.To compare the effect of different chemical agents between different types ofmicroorganisms.3.To compare the effect between physical agents and chemical agents in controllingmicrobial growth.INTRODUCTION:Soil microbiology is the study of microorganisms in the soil and their functionswhich contribute to the fertility of the soil. Most of the soil microorganisms are found in theupper layer of soil which contained high organic matter that favour for the microbial activity.Among the microorganisms found in soil, few of them are able to synthesize antibioticswhich to inhibit the growth of other microorganisms. The four groups of antibiotic producingmicroorganisms which have been isolated from soil are Streptomyces, Bacillus, Penicilliumand Acremonium. These represent the three types of microbe which are actinomycetes, truebacteria and mold (Hoorman, 2016).Actinomycetes are one of the member of a heterogeneous group of gram-positiveand anaerobic bacteria. Although they are grouped under the Bacteria kingdom, but theyshare some characteristic with fungi. It mostly found in a form of mycelium in a filamentousand branching growth pattern. Most of the species of actinomycetes are found in the soilwhich are harmless to animals but some are the pathogen to the plants. One of the beneficialcharacteristic of actinomycetes is the ability to produce antibiotics which is the secondarymetabolites produced by actinomycetes that are important in the medical research(Encyclopaedia Britannica, 2018). The example of antibiotic produced is streptomycin whichable to inhibit the microbial growth through binding to the 30S ribosomal subunit result in theinhibition of the synthesis and causing misreading of mRNA. The common use ofstreptomycin in treatment of tuberculosis (Garg, 2015).Soil bacteria are important in the soil ecosystem as they play a vital in biochemicalprocess such as nitrogen cycle. Through the activity carried out by the bacteria, the soilquality is improved and increase the nutrient content in soil. The dilution and plating of soilbacteria is applied in enumeration of bacteria in the sample soil. By enumerating the bacteria2
in the soil, it will give an information of the soil health (JoVE Science Education Database,2018).

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